PART1 m6A/MeRIP-seq

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手机游戏开发者 2024-10-8 06:21:56 646 0 来自 中国
此次学习的m6A/MeRIP-seq 得当单个细胞样本大概bulk样本

work flow

1.png 须要用到的包:

Cutadapt, tested with 1.15
Samtools, tested with 1.7
HISAT2, tested with 2.1.0
Picard, tested with 2.17.10
bedtools, tested with 2.27.1
bedGraphToBigWig
StringTie, tested with 1.3.4d
prepDE.py
gffcompare, tested with 0.10.4
MACS2, tested with 2.1.1.20160309
HOMER, tested with 4.9
R, tested with 3.3.3. Install three libraries exomePeak, MeTPeak and MeTDiff in R.
参考文档:shunliubio/easym6A: Process m6A/MeRIP-seq data in a single or batch job mode (github.com)
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